Candidates searching for BTEUP Syllabus of UP Diploma/Polytechnic must take a look here. The Board Of Technical Education Uttar Pradesh Lucknow conducts various UP Diploma/Polytechnic courses and after each semester or year the institution conducts exam to check the intelligence and learning level of candidates. The course content of courses conduct is always based on the BTEUP Syllabus 2018. Candidates pursuing the foresaid program and willing to get the Sem Wise Syllabus of All branches must take a glance over this whole page.
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CURRICULUM FOR ONE YEAR (TWO SEMESTERS) P.G. DIPLOMA COURSE IN BIOTECHNOLOGY (TISSUE CULTURE)
- Analytical Biochemistry
- Molecular Biology and Genetic Engineering
- Industrial Microbiology
- Plant Tissue Culture Technology
- Games/NCC/Social and Cultural Activity Community Development Work+Discipline
- Green House Technology
- Environmental Education(*)And Disaster Management
- Project Work
- Games/NCC/Social and Cultural Activity Community Development Work+Discipline
(1) Each period will be of 50 minutes duration.
(2) Each session will be of 16 weeks.
(3) Effective teaching will be at least 14 weeks.
(4) Remaining periods will be utilized for revision etc.
(5) Structured and supervised task oriented project work to be organized during teaching. Student will submit a report there will be 200 marks for this project. Each marks will be awarded by the examiner (examination Marks 120 : Sess. Marks 80)
(6) Environment Awarness Camp: To be organized at a stretch for 3 days. No Examination. Entrepreneurship Camp: To be organized at a stretch for 1 week No examination.
(7) (*) It is compulsory to appear & to pass in examination, But marks will not be included for division and percentage of obtained marks.
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Now let’s take a look over the detailed syllabus:
1.1 ANALYTICAL BIOCHEMISTRY
Basic knowledge about Analytical Biochemistry is essential for the students of diploma in Biotechnology (Tissue Culture). The elements of biomolecules, carbohydrate metabolism, lipid metabolism, enzymes, nitrogen metabolism, photosynthesis and physiology are important part of biochemistry which shall be taught to the students through this subject besides introduction about spectrophotometry, microscopes etc.
1. General principles of analytical biochemistry.
|2. The concept of pH, Properties of water, dissociation and ionization of acids and bases, pKa, buffers adn buffering mechanisms, dissociations of amino acids and determination of pKa.|
|3. Structure of proteins and metabolism, carbohydrates and lipids.|
|4. Chromatography: principles and application of adsorption, partition, ion-exchange, gel filtration, affinity and high performance liquid chromatography.|
|5. Electrophoresis : moving boundry and zonal electrophoresis, paper and gel electrophoresis, isoelectric focusing technique.|
|6. Centrifugation techniques and application.|
|7. Electroanalytical methods.|
|8. Principles and applications of ultraviolet and visible light spectrophotometry, NMR spectroscopy, spectroflurmetry and atomic spectroscopy, mass spectroscopy.|
9. Tracer technices, ELISA, RIA, IRMA, Scintillation counters.
LIST OF PRACTICALS
- To prepare various buffer solutions required for tissue culture and bio-chemical work.
- Separation of leaf pigment adsorption chrnatigraogy.
- Seperation of amino acids by TLC.
- Qualitative and quantitative analysis of carbohydrates.
- Determination of acid value of fat.
- Determination of iodine number of fat.
- Qualitative and quantitative analysis of proteins.
- Estimation of Enzyme activity.
- Protein purification by gel filtration chromate graphy.
- Molecular weight determination of protein through SDS PAGE.
- Centrifugation and separation of cell components.
1.2 MOLECULAR BIOLOGY AND GENETIC ENGINEERING
The course will deal with the knowledge of types of cells, composition, function and genome organization of various cell types and an acquaintance with manipulation of DNA.
- Introduction, Development, Present Status and Prospects of Mocular Biology and Genetic Engineering.
- Replication of DNA,Types, Structure and properties of DNA and RNA and the genetic code.
- Genome organization in viruses, Prokrayotes and eukaryotes.
- Central Dogma,Transcription, Translation, Regulation of Gene expression.
- A brief account of repetitive DNA sequences, split genes, transposons.
- Introduction to gene mapping, Genomics and Metabolomics, ionomics , phenomics and proteomics.
- Fundamentals of recombinant DNA technology: DNA extraction, labelling of nucleic acids, Restriction endonucleases, cloning vectors, Isolation and cloning of genes,gene libraries.
- Fundamentals of molecular techniques and their uses PCR, Gel documentation and Blotting techniques.
- Isolation of plasmid DNA from E. Coli. (Transformed) cells.
- Quantitation of nucleic acids by gel electrophoresis and spectrophotometry.
- Isolation of DNA from plant and microbial cells.
- Spectrophotometric Determination of melting temperature of DNA.
- Demonstration of digestion of DNA by restriction endo nuclease and agarose gel electrophoresis.
- Isolation of RNA.
- Mutation in yeast by physical multagenic agents.
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1.3 INDUSTRIAL MICROBIOLOGY
In order to exploit medicinal and industrial application of cellular processes in tissue culture it is essential to undertstand the basic concepts of cell structure, function and interactions in microbes. This subject has been designed giving more emphasis on fundamentals of various principles involved. Students will be able to utilize their skill in the application of microbial techniques towards commercial application.
- Microbial growth and nutrition, Fermentation Media.
- Microbial metabolism, Glycolysis, TCA and Secondary metabolic path ways.
- Classes and properties of micro-organisims relevant to water, food, alcohal, dairy bio fertilizer and bio opertative industries etc.Industrial microorfanisms, important for water, food alcohol, dairy, biofertilizer biopesticide industries, etc..
- Fermentation systems, Aerobic and anaerobic, Solid and submerged, Surface culture.
- Bioreactors- types and uses. Downstream processing-step and methods.
- Microbial enzymes, Protease, Amylates, Lipates and their application.
- Fuels and industrial products Ethanol, Acetone, Butanol.
- Health care products, Therapeutic and Antibiotics.
- Food and beverages fermentaions Systems.
- Food additives, Vitamins and Aminoacids.
- Waste water treatment : Characterization techniques and Methods.
LIST OF PRACTICALS
- Sterilization of glasswares and preparation of culture media for microbial growth.
- Simple and differential staining of different micro- organisms.
- Microbial growth measurement by different methods : (Counting, O D., measurement).
- Effect of pH, temperature and substrate conc. on growth of microorganisms.
- Fermentative production of alcohol.
- Determination of total dissolved solids of water.
- Determination of biological oxygen demand (BOD) of water.
- Determination of chemical oxygen demand (COD) of water.
- Calony characterzation of micro organisms.
- Microscopic determination of shape and size of yeast cell.
- Screening and isolation of Azato bacter.
- Maintenance of culture on agar medium.
- Production of invertate enzyme.
1.4 PLANT TISSUE CULTURE TECHNOLOGY
Since the students will be engaged in the use of Tissue Culture in biotechnology, the fundamentals of Tissue Culture Technology will help them to gain knowledge and skill in Plant Tissue Culture which can be used for improvement of varities of crops, e.g., grain quality, fibre quality, disease resistance insect resistance, herbicide resistance etc.
- Introduction: Definition, history, present status, future prospects of tissue culture, Cellular totipotency.
- Composition of different culture Media, Role of chemicals and growth regulators in tissue culture.
- Sterilization of media, tissues and other accessories, Inoculation of cultures.
- Callus Induction and plant regeneration. Organogenesis and Embryogensis.
- Suspension culture, Protoplast isolation, culture and fusion.
- Clonal propagation : Shoot-tip and axillary bud culture.
- Techniques of cell and tissue culture: Preparation of explant materials, initiation of cultures, micropropagation through organogensis and embryogensis, artificial seeds.
- Anther Culture: Development of haploids, diploidization and its applications.
- Embryo culture.
- Somaclonal variations and in vitro selections towards crop improvement.
- Industrial appliction of plant tissue culture for production of Secondary metabolites.
- Plant biotechnology and crop improvement for grain quality, fiber quality, disease resistance, insect resistance, herbicide resistance, stress resistance (basic outlines).
- Orientation : Introduction to tissue culture laboratory; washing chamber, media preparation laboratory, transfer laboratory, sterlization laboratory inoculation laboratory, culture room.
- Tissue culture requirements; glass ware, water distillation Unit, chemicals, instruments; Autoclave, pH meter, sterile air flow chamber (Laminar flow).
- Preparation of Media.
- Sterlization: Media, Explant,glassware.
- Inoculation, Callus Induction and clonal propagation.
- Subculturing and Regeneration of roots, shoots and plants.
- Preparation of regenerated plants for hardening.
- Protoplast Isolation and culture(demonstration).
- Induction of embryogenic callus and encapsulation of artificial seeds.
- Anther culture: Regeneration of plants from anthers/pollens.
- Agrobacterium – mediated transformations (demonstration).
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2.1 GREEN HOUSE TECHNOLOGY
After passing the programme the students will have to work in green house or some of them may opt to start their own green house. Appropriate knowledge and relevant skills are necessary in order to establish and maintain the green houses. The course will help to plan and fabricate suitable green house with controlled environmental parameters for different climatic zones to harden tissue culture derived plants and grow high value ornamentals, cut flowers and vegetables to meet market demand in off season and, export.
- Environmental parameters for optimum plant growth.
- Photoperiodism, vernalization.
- Green House and polyhouse materials, designs & fabrication.
- Cooling, shading, misting, drip irrigation,fertilization and fumigation.
- Recording and Computerisation of environmental parameters and cultural operations.
- Pots, potting mixtures and soil sterilization.
- Cultivation of Horticultural plants.
- Hardening of tissue culture derived horticultural plants.
- Post harvest technology.
- Packaging and transportation.
- Intellectual Property Rights.
- Raising of off-season nursery in poly houses.
- Preparation of tissue culture derived plants for hardening.
- Preparation of soil mix and sterilization.
- Hardening of tissue culture derived plants.
- Soil culture and hydroponics, sand culture of roses and selected vegetables in green house.
- Harvesting, preparation, cooling of cut flowers and vegetables.
- Packing and transportation (Demonstration only).
2.2 ENVIRONMENTAL EDUCATION & DISASTER MANAGEMENT
A diplima student must have the knowledge of different types of pollution caused due to industrialisation and construction activities, so as he may help in balancing of eco-system and control pollution by providing controlling measures. They should be also aware of the environmental laws for effectively controlling the pollution of environment. The topics are to be taught in light of legislation Para-3.
- INTRODUCTION : – Basics of ecology, Ecosystem, Biodiversity Human activities and its effect on ecology and eco system, different development i.e. irrigration, urbanization, road development and other engineering activities and their effects on ecology and eco system, Mining and deforestation and their effects. – Lowering of water level , Urbanization. – Biodegradation and Biodegradibility, composting, bio remediation, Microbes .Use of biopesticidies and biofungicides. – Global warning concerns, Ozone layer depletion, Green house effect, Acid rain,etc.
- POLLUTION : Sources of pollution, natural and man made, their effects on living environments and related legislation.
WATER POLLUTION :
- Factors contributing water pollution and their effect.
- Domestic waste water and industrial waste water. Heavy metals, microbes and leaching metal.
- Physical, Chemical and Biological Characteristics of waste water.
- Indian Standards for qulity of drinking water.
- Indian Standards for quality of treated waste water.
- Treatment methods of effluent (domestic waste water and industrial/ mining waste water), its reuse/safe disposal.
AIR POLLUTION :
- Definition of Air pollution, types of air pollutants i.e. SPM, NOX, SOX, GO, CO2, NH3, F, CL, causes and its effects on the environment.
- Monitoring and control of air pollutants, Control measures techniques. Introductory Idea of control equipment in industries i.e.
- Settling chambers
- Scrubbers (Dry and Wet)
- Multi Clones
- Electro Static Precipitations
- Bog Fillers.
- Ambient air qulaity measurement and their standards.
- Process and domestic emission control
- Vehicular Pollution and Its control with special emphasis of Euro-I, Euro-II, Euro-III and Euro IV.
NOISE POLLUTION : Sources of noise pollution, its effect and control.
RADISACTIVE POLLUTION : Sources and its effect on human, animal, plant and material, means to control and preventive measures.
SOLID WASTE MANAGEMENT : Municipal solid waste, Biomedical waste, Industrial and Hazardous waste, Plastic waste and its management.
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Preliminary knowledge of the following Acts and rules made there under-
- The Water (Prevention and Control of Pollution) Act – 1974.
- The Air (Prevention and Control of Pollution) Act – 1981.
- The Environmental Protection (Prevention and Control of Pollution) Act -1986. Rules notified under EP Act – 1986 Viz.
# The Manufacture, Storage and Import of Hazardous Chemical (Amendment) Rules, 2000
# The Hazardous Wastes (Management and Handling ) Amendment Rules, 2003.
# Bio-Medical Waste (Management and Handling) (Amendment) Rules, 2003.
# The Noise Pollution (Regulation and Control) (Amendment) Rules, 2002.
# Municipal Solid Wastes (Management and Handling) Rules, 2000.
# The Recycled Plastics Manufacture and Usage (Amendment) rules, 2003.
ENVIRONMENTAL IMPACT ASSESSMENT (EIA):
- Basic concepts, objective and methodology of EIA.
- Objectives and requirement of Environmental Management System (ISO-14000) (An Introduction).
Definition of disaster – Natural and Manmade, Type of disaster management, How disaster forms, Destructive power, Causes and Hazards, Case study of Tsunami Disaster, National policy- Its objective and main features, National Environment Policy, Need for central intervention, State Disaster Authority- Duties and powers, Case studies of various Disaster in the country, Meaning and benifit of vulnerability reduction, Factor promoting vulnerability reduction and mitigation, Emergency support function plan.
Main feature and function of National Disaster Management Frame Work, Disaster mitigation and prevention, Legal Policy Frame Work, Early warning system, Human Resource Development and Function, Information dissemination and communication
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2.3 PROJECT WORK
The purpose of introducing project work is to enable the students to apply the knowledge, skills and attitudes, acquired during the entire course, to the solution of specific problems related to practical world of work. It is expected that students will be sent to various organisations at a stretch for 8 weeks and be asked to take up any live problem of the organisation as project work. Students may also be given option to take up entrepreneurial projects and projects related to environment/ ecology and waste management/utilisation. The project identification should be done by the students in consultation with teachers and personnel from the organisations where the student is going to undertake the project activity on the basis of basic principle; “problem needing to immediate solution”. Each teacher should guide 3 – 4 students for project work throughout this activity. The students will have to go through all the steps of problem solving such as defining the problem, analysis of the problem, collection of required information and resources, formulating alternatives, selecting best solution and reduction in practice.
The project work aims at, besides developing problem solving abilities in the students, development of confidence and expertise in a particular field. The student may get training to analyse the problem, use of instruments, use of techniques and orientation of learning experiences towards their applications in the world of work. Some of the suggested projects are given below:
PLANT TISSUE CULTURE
Initiation of a culture and finally production of plant in tissue culture system
These are only some of the suggestive projects, infact, students can totally ignore this list and identify projects with the help of their teachers/personnel from industry/organisation.
Students will be assessed on the basis of the project report and viva voce examination by internal and external examiners on the pattern suggested below:
1. Panctuality and regularity
|2. Level/Proficiency of Practical Skills Demonstrated||25 percent|
|3. Sense of initiative and responsibility||10 percent|
|4. Interpersonnel skills/Human Relations/ Behaviour during the project work||10 percent|
|5. Report Writing||25 percent|
|6. Viva Voce||15 percent|
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2.4 ENVIRONMENTAL AWARENESS CAMP
A post diploma holder in Bio-technology (Tissue Culture) must have the knowledge of different types of pollution caused due to industrialisation and construction activities so as he may help in balancing the eco system and control pollution by providing controlling measures. He should be also aware of the environemntal laws for effectively controlling the pollution of environment.
This is to be organised at a stretch for two to three days. Lectures will be delivered on the following broad topics. There will be no examination for this subject.
- Basics of ecology, eco system and sustainable development
- Conservation of land forms, preservation of species, prevention of advancements of deserts and lowering of water table.
- Sources of pollution – Natural and man made, their effects on living and non-living organisms
- Pollution of water – causes, effects of domestic wastes and industrial effluent on living and non living organisms
- Pollution of air – causes and effects of man, animal, vegetation and on non-living organisms
- Sources of noise pollution and its effects
- Mining, blasting, deforestation and their effects
- Legislations to control environment
- Environmental Impact Assessment (EIA) Elements for preparing EIA statement.
ENTREPRENEURSHIP AWARNESS CAMP
Besides finding employment in any organization there is a great scope for the students of post diploma in Biotechnology (Tissue Culture) to get self employed and establish their own units. For this purpose knowledge about feasibility report based on the market research, financial and other institutions which can provide different types of support/assistance to the entrepreneurs and management is very essential. It is proposed to organise an entrepreneurship awareness camp for one week at a stretch for the students toward the end of academic session.
- Concept of entrepreneurship, need and development of entrepreneurial attitudes and qualities
- Market research and feasibility report – methods for market research and preparation of feasibility report
- Basic resources finance, infrastructure, manpower and training, technology and information resources – their sources, prominent procedure and budgeting etc *
- Assistance available from District Industries Centres, Commercial Banks, State Financial Corporation, Small Industries Service Institute, Research and Development Laboratories/Organisations and other Financial and Developmental Organisations
- Book keeping and material inventory control 6. Quality management, sales and marketing techniques
NOTE: The students should be given at least one exercise for preparation of feasibility report for any product.
* Experts from various organisations/Institutions may be invited to explain the type of facilities available and procedures for availing these facilities from their prospective organisations/institutions
Download here>> BTEUP Syllabus for Bio Technology PDF
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